Molecular Diagnostic PCR Handbook - Gerrit J Viljoen - Livros - Springer-Verlag New York Inc. - 9781402034039 - 19 de julho de 2005
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Gerrit J Viljoen
Molecular Diagnostic PCR Handbook 2005 edition

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Provides an account of the practical aspects of PCR. This work includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. It explains the difficulties, advantages and disadvantages in PCR applications.

Marc Notes: Includes bibliographical references and index. Table of Contents: Preface. Acknowledgements. Chapter One: Background. Chapter Two: PCR the basic reaction. Chapter Three: Amplicon detection, analysis and PCR evaluation. Chapter Four: Limitations and Troubleshooting. Chapter Five: Specific types of PCR assays. Chapter Six: The PCR laboratory. Chapter Seven: Disease diagnosis using PCR specific procedures for important veterinary pathogens. Appendices. Technical. Information and general protocols. Appendix A: Thermal cyclers. Appendix B: Basic protocol for agarose gel electrophoresis. Appendix C: Using nucleic acid hybridisation in order to verify the integrity of a PCR amplicon. Appendix D: Digestion of DNA with restriction endonucleases and electrophoresis of the restriction fragments. Appendix E: DNA sequence analysis. Appendix F: An example of a RT-PCR protocol. Appendix G (i): Calibration and Maintenance of Mechanical Pipettes. Appendix G (ii): Standard Operating Procedure for using micropipettes. Appendix H: Standard Operating Procedure for using clocks and timers. Appendix I: Theoretical background to pH, buffers and concentration of solutions. Appendix J: General buffer and stock solutions. Appendix K: General reagents and suppliers. Appendix L: Bioinformatics. Original Papers. Glossary. Index."Publisher Marketing: Provides an account of the practical aspects of PCR. This work includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. It explains the difficulties, advantages and disadvantages in PCR applications.

Contributor Bio:  Crowther, John R Crowther, Institute for Animal Health, Pirbright Laboratory, Waking, UK.

Mídia Livros     Hardcover Book   (Livro com lombada e capa dura)
Lançado 19 de julho de 2005
ISBN13 9781402034039
Editoras Springer-Verlag New York Inc.
Páginas 308
Dimensões 156 × 232 × 19 mm   ·   635 g
Editor Crowther, John R.
Editor Nel, Louis H.
Editor Viljoen, Gerrit J.

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